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CBSE Class 12 Biotechnology Sample Paper: 2018

Nov 13, 2017 15:24 IST
CBSE Sample Papers 2018 for Class 12 Biotechnology
CBSE Sample Papers 2018 for Class 12 Biotechnology

Download CBSE Sample Paper 2018 for 12th Biotechnology (Theory) in PDF format. Links to download CBSE 12th Biotechnology (Theory) Sample Paper 2018 and its Marking Scheme are given at the end of this article.

CBSE Class 12 Biotechnology Sample Paper 2018 is released by CBSE along with Marking Scheme and hints. With the help of this CBSE Sample Paper, students of Class 12 will get an idea about the latest examination pattern and the level of questions which can be asked in the coming CBSE class 12 Biotechnology board exam 2018.

Students must solve this Sample Paper & refer their solutions with the Marking Scheme to assess their preparation level of CBSE class 12 Biotechnology (Theory) board exam 2018. All the questions of this CBSE Sample Paper are very important for the coming Class 12th Biotechnology board exam 2018.

CBSE Class 12 Biotechnology Syllabus 2017 – 2018

Complete CBSE Class 12 Biotechnology Sample Paper 2018 is given below:

Sample Question Paper

Class XII

Biotechnology (Theory)

Subject Code: 045

Time: 3 hours                                                                                                  Max. Marks:  70

General Instructions:

•All questions are compulsory

•Question paper consists of 4 sections A, B, C and D

•Question numbers 1 to 6 are very short answer questions each carrying one mark

•Question numbers 7 to 14 are short answer questions each carrying two marks

•Question numbers 15 to 25 are also short answer questions each carrying three marks

•Question numbers 26 to 28 are long answer questions each carrying five marks

•There is no overall choice. However an internal choice has been provided in one question of three marks and two questions of five marks. You have to attempt only one of the choices in such questions.

•Use of calculators is not permitted .However, you may use log tables, if necessary.

Section A

Question 1:

Specify the role of “cos” sites in bacteriophage lambda.

Question 2:

Expand and define PER.

Question 3:

What would be the effect of an aqueous environment on the bond strength of ionic bonds between amino acid residues in a protein?

Question 4:

How is lipofection used to deliver genes into cells?

Question 5:

Name the scientists who were first to introduce trypsin for the sub culturing of adherent cells.

Question 6:

Write any one distinguishing feature of pBR 322 and pUCI9 vectors.

Section B

Question 7:

What is meant by tissue engineering?

Question 8:

How does the metagenomics approach help to identify novel genes present in the environment? Explain the process.

Question 9:

Explain various plant regeneration pathways.

Question 10:

A researcher wants to introduce a desired gene into a specific host cell .Write any two methods that can be used for the same.

Question 11:

Differentiate between somaclonal variations and gametoclonal variations. Who proposed the term “Somaclones” for plant variants?

Question 12:

C.e!egans a eukaryotic organism with a genome of 97 Mb and about 20,000 genes. Why does organizational feature of this genome are unusual when compared to the genomes of other eukaryotes, such as yeast and Drosophila?

Question 13:

Highlight graphically the differences between culturing microbes in the school laboratory and a bioreactor which allow cells to grow in a continuous culture system.

Question 14:

How can you maximize protein stability during purification? Write any two parameters for the same.

Section C

Question 15:

Discuss the various types of shapes & structures that a protein takes to make a functional protein .Write various forces responsible for these structures.

Question 16:

Outline the process of creation of chimeric mouse by embryonic stem cell culture.

Question 17:

What is a DNA probe? Explain the principle of Sanger’s method of DNA sequencing.

Question 18:

Expand BLAST. Differentiate between Homologues and Paralogs.

Question 19:

You have succeeded in purifying a protein from yeast .Name a technique you would use and the principle behind it for determining the molecular mass of this protein.

Question 20:

Explain the methods which can be used for the scaling up of animal culture.

Question 21:

State any three advantages of using Pichia pastoris as a eukaryotic expression host.

Question 22:

How would you detect a specific microbial contamination from a given water sample using PCR. Give a brief explanation of the process.

Question 23:

Following are few transgenic crops approved by U.S.F.D.A. identify 'a’, 'b’ and ‘c’ and complete the table.

CBSE Class 12 Biotechnology Sample Paper: 2018 - Question 23

Question 24:

Rohan cultured streptococcus bacteria in his lab to check whether it is gram positive or negative and then he threw the culture directly in dustbin. Is this method of disposal ethically and ecologically safe?

Question 25:

What are edible vaccines? How are they better than conventional vaccines? Give any two points.


What are somatic hybrids? How are they produced?

Section D

Question 26:

What are type II restriction endonucleases? Give an example of a type II restriction endonucleases that generates flush ends and the sequence recognized by it. Explain how are they named. Name any other enzyme and its utility in cloning experiment.

Question 27:

Name the technique developed by O’ farrel. Schematically depict key steps in the separation of proteins using the technique. Highlight the basis of separation at each step.


Classify protein based products. Give one example under each category along with its application. How are these useful to the biotechnology industry?

Question 28:

Explain how cDNA microarray technique can be used to study cellular response to the environment? Support your answer with a flowchart for the same.


(a) Which information can be retrieved from the following databases?

(i) EMBL

(ii) PDB

(iii) PALI

(b) Give two reasons for completely sequencing a genome.


CBSE Class 12 Biotechnology Sample Paper: 2018 in PDF format

Hints (or Marking Scheme) of CBSE Class 12 Biotechnology Sample Paper: 2018 in PDF format

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