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CBSE Class 12 Biotechnology Syllabus 2019-20

Latest syllabus of CBSE Class 12 Biotechnology subject is available here. With the help of this article, you can also download this syllabus in PDF format.

May 21, 2019 18:25 IST
    CBSE Class 12 Biotechnology Syllabus
    CBSE Class 12 Biotechnology Syllabus

    CBSE Syllabus for Class 12 Biotechnology subject is available here for download in PDF format. Link to download CBSE Class 12 Biotechnology Syllabus 2019-20 is given at the end of this article. Besides the syllabus, here you will also get complete details about practical is CBSE Class 12 Biotechnology subject for the session 2019-20.

    Important portion of CBSE Class 12 Biotechnology Syllabus 2019-20 is given below

    Units

     

    No. of Periods

    Marks

    V

    Protein and Gene Manipulation

    100

    40

    VI

    Cell Culture and Genetic Manipulation

    80

    30

     

    Practicals

    60

    30

     

    Total

    240

    100

    Unit-V Protein and Gene Manipulation (40 Marks)

    Chapter-1: Recombinant DNA Technology

    Introduction, Tool of DNA technology, Making DNA, Introduction of recombinant DNA into host cells, Identification of Recombinants, Polymerase Chain Reaction (PCR), Hybridization Techniques, DNA Library, DNA Sequencing, Site-directed Mutagenesis

    CBSE Class 12 Syllabus 2019-20: All Subjects

    Chapter-2: Protein Structure and Engineering

    Introduction to the world of proteins, 3-D shape of proteins, Structure-Function Relationship in Proteins, Purification of Proteins, Characterization of Proteins, Protein Based Products, Designing Proteins (Protein Engineering)

    Chapter-3: Genomics, Proteomics and Bioinformatics

    Introduction, Genome, Sequencing Projects, Gene prediction and counting, Genome Similarity, SNPs and

    Comparative Genomics, Functional Genomics, Proteomics, History of Bioinformatics, Sequences and nomenclature, Information Sources, Analysis using Bioinformatics tools

    Unit-VI Cell Culture and Genetic Manipulation (30 Marks)

    Chapter-1: Microbial Cell Culture and its Applications

    Introduction, Microbial Nutrition and Culture Techniques, Measurement and Kinetics of Microbial Growth, Scale-up of Microbial Process, Isolation of Microbial Products, Strain Isolation and Improvement, Applications of Microbial Culture Technology, Biosafety Issues in Microbial Technology

    Chapter -2: Plant Cell Culture and Applications

    Introduction, Cell and Tissue Culture Techniques, Applications of Cell and Tissue Culture, Gene Transfer Methods in Plants, Transgenic Plants with Beneficial Traits, Biosafety of Transgenic Plants

    Chapter-3: Animal Cell Culture and Applications

    Introduction, Animal Cell Culture Techniques, Characterization of Cell lines, Methods of Gene Delivery into Cells, Scale-up of Animal Culture Process, Applications of Animal Cell Culture, Stem Cell Technology, Tissue Engineering

    Syllabus of CBSE Class 12 Biotechnology Practicals 2019-20 (30 Marks)

    Note: Every student will be required to do the following experiments during the academic session.

    1. Use of special equipment in biotechnology experiments

    2. Isolation of bacterial plasmid DNA

    3. Detection of DNA by gelelectrophoreses

    4. Isolation of Genomic DNA (CTAB method)

    5. Estimation of DNA

    6. Bacterial transformation using any plasmid

    7. Restriction digestion of plasmid DNA & its analysis by gelelectrophoresis

    8. Isolation of bacteria from curd & staining of bacteria

    9. Cell viability assay

    10. Data retrieval and database search using internet site NCBI and download a DNA and protein sequence from internet, analyze it and comment on it

    11. Reading of a DNA sequencing gel to arrive at the sequence

    12. Project work

    Scheme of Evaluation: Practicals (Time: 3 Hours, Max. Marks 30)

    A

    Two Experiments

    6+6 (only one computer based practical)

     

    Practical Record

    04

     

    Viva on Practical

    04

    B

    Project Work

     

     

    Write up

    05

     

    Viva on project

    05

     

     

    30

    Prescribed Books for CBSE Class 12 Biotechnology:

    • A Text Book of Biotechnology - Class XII: Published by CBSE, New Delhi

    • A Laboratory Manual of Biotechnology - Class XII: Published by CBSE, New Delhi

    Download CBSE Class 12 Biotechnology Syllabus 2019-20 in PDF Format

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