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CBSE Class 12 Biotechnology Syllabus 2017 – 2018

Apr 19, 2017 17:10 IST

CBSE Class 12 Biotechnology Syllabus 2017 – 2018

CBSE 12th Biotechnology Syllabus 2017 - 2018 is available here for download in PDF format. It contains details about course structure, practicals & question paper design.

Important portion of CBSE Class 12 Biotechnology Syllabus 2017 - 2018 is given below


No. of Periods


Unit V. Protein and Gene Manipulation



Unit VI. Cell Culture and Genetic Manipulation









Detail of topics and subtopics in each unit

Unit-V Protein and Gene Manipulation

Chapter-1: Recombinant DNA Technology Introduction, Tools of Recombinant DNA technology, Making Recombinant DNA, Introduction of Recombinant DNA into Host Cells, Identification of recombinants, Polymerase chain reaction (PCR), Hybridization techniques, DNA library, DNA sequencing, Site-directed mutagenesis

Chapter-2: Protein Structure and Engineering Introduction to the World of Proteins, 3-D Shape of Proteins, Structure-function relationship in Proteins, Purification of proteins, Characterization of proteins, Protein Based products, Designing proteins (protein engineering)

Chapter-3: Genomics, Proteomics and Bioinformatics Introduction, Genome sequencing projects, Gene prediction and Counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, History of bioinformatics, Sequences and Nomenclature, Information sources, Analysis using bioinformatics Tools

CBSE Class 12 Mathematics Syllabus 2017 – 2018

Unit-VI Cell Culture and Genetic Manipulation 30 Marks 80 Periods

Chapter-1: Microbial Cell Culture and Its Applications

Introduction, Microbial culture techniques, Measurement and kinetics of microbial growth, Scale-up of microbial process, Isolation of microbial products, Strain isolation, improvement and Preservation, Applications of microbial culture technology, Biosafety Issues in microbial technology.

Chapter-2: Plant Cell Culture and Applications

Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Gene transfer methods in plants, Transgenic plants with beneficial traits, Biosafety of Transgenic Plants.

Chapter-3: Animal Cell Culture and Applications

Introduction, Animal cell culture techniques, Characterisation of cell lines, Methods of gene delivery into cells, Scale-up of animal culture process, Applications of animal cell culture, Stem cell technology, Tissue engineering.

CBSE Class 12 Biology Syllabus 2017 - 2018

Practicals (30 Marks)

Note: Every student will be required to do the following experiments during the academic session.

List of Experiments

1. Use of special equipment in biotechnology experiments.

2. Isolation of bacterial plasmid DNA

3. Detection of DNA by gel electrophoreses

4. Isoloation of Genomic DNA (CTAB method)

5. Estimation of DNA

6. Bacterial transformation using any plasmid

7. Restriction digestion of plasmid DNA & its analysis by gel electrophoreses

8. Isolation of bacterial grom curd & staining of bacteria

9. Cell viability assay

10. Data retreival and data base search using internet site NCBI and download a DNA and protein sequence from internet, analyse it and comment on it

11. Reading of a DNA sequencing gel to arrive at the sequence

12. Project work

Scheme of Evaluation (Practicals):

Time 3 Hours | Maximum marks 30


Two experiments

6+6 (only one computer based practical)


Practical record



Viva on Practicals



Project work



Write up



Viva on project





Prescribed Books:

1. A Text Book of Biotechnology - Class XII : Published by CBSE, New Delhi

2. A Laboratory Manual of Biotechnology - Class XII : Published by CBSE, New Delhi

Download CBSE Class 12 Biotechnology Syllabus 2017 – 2018

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