The Central Institute for Research on Buffaloes (CIRB) on March 16, 2018 claimed that a cloned Assamese buffalo male calf was born for the first time in India.
A team of researchers used unique methods and produced the clone calf 'Sach-Gaurav' on December 22, 2017 through a normal delivery. The CIRB cloning team was led by PS Yadav.
'Sach-Gaurav' is the second cloned male produced by CIRB after Hisar-Gaurav, born on December 11, 2015. Hisar-Gaurav was producing quality semen from the age of 22 months and ten females of the institute's herd were pregnant with its frozen semen.
Assamese buffalo Sach-Gaurav
• The calf was born to a Murrah buffalo.
• It is also the first to be born in the field, which is 100 km away from the cloning laboratory at Hi Tech Sach Dairy Farm in Hisar.
• The birth weight of the calf was 54.2 kgs and it was healthy.
• It had normal physiological parameters and blood profile.
• The genotype of the calf was confirmed through microsatellite analysis (parentage verification) and chromosome analysis.
• The cloned embryos were transported for about two hours before being transferred into the recipient mothers.
• The ovaries of Murrah buffaloes were used as a source of recipient oocytes.
• Small tissue of Assamese buffalo was airlifted from Veterinary College, Khannapara, Guwahati, which is 2000 km away from cloning laboratory to culture establish the donor cells.
Assamese buffaloes are found only in the North-Eastern part of the country and they are used mainly for agricultural work. However, through cloning, it will not be restricted to a particular region now.
Animal cloning can be an excellent reproductive tool for conservation and multiplication of selected superior animals of buffalo breeds.