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Biotechnology: Principles and Processes-Important Questions & Preparation Tips

In this article you will get the clue that from where and how the questions are being framed from the chapter Biotechnology: Principles and Processes

Apr 22, 2013 15:41 IST

In this article you will get the clue that from where and how the questions are being framed from the chapter Biotechnology: Principles and Processes
The experts of jagranjosh.com have full confidence that you will not miss any of the questions in the board exam as well as in competitive exams from this chapter if you will keep the following terms / question in your mind while studying.

• Difference between traditional and modern biotechnology

• What is conventional biotechnology?

• Advantages of GMO,s

• What are DNA vaccines?

• What is IVF?

• What is EFB?

• What is recombinant DNA technology?

• How first rDNA was developed?

• At what stage of meiosis a recombinant DNA is made?

• Bring out the disadvantages in conventional hybridization as compare to rDNA technology

• Give an example of palindromic DNA sequence

• What is a plasmid?

• Class of restriction enzyme to which it belongs

• Why restriction enzyme is given the name restriction?

• What is restriction modification synthesis

• Name the first endonuclease and it source

• Naming convention of E.coli

• Name any four restriction enzyme

• Substance used as a matrix in gel electrophoresis

• Define electrophoresis

• Compound used to stain the isolated DNA

• Define ‘ori’

• Define microinjection and gene gun method

• Charge on DNA

• What is the two sets of primers needed for amplification of gene

• Why Taq polymerase is needed in amplification or in genetic engineering?

• What are advantages of sparged stirred-tank bioreactor?

• Define sampling ports in bioreactor

• What is bioconversion?

• Deference between gene therapy  and gene cloning

• Selection markers and their advantages

• How is the gene z(for β galactosidase)used as a marker

• How a host cell is made competent in introducing recombinant DNA?

• What is continuous culture?

• Major difference between simple stirred tank bioreactor and sparged stirred tank bioreactor? What is its advantages

• Recognition sequence

• E.Coli cloning vector β PBR322

• Short note on GEAC

• Function of all enzymes used in this chapter

• Diagrammatic representation to construct recombinant DNA

• Detail study of PCR

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