CBSE Class 12 Biotechnology Sample Paper 2025-26, Download Model Paper PDF FREE

Section - A 

Q. No. 1 to 12 are multiple choice questions. Only one of the choices is correct. Select and write the correct choice as well as the answer to these questions. 

Q. No.

Questions

Marks

1.

In which phase of growth, specific growth rate of microbial culture is calculated? 

(a) Lag phase 

(b) Exponential phase 

(c) Stationary phase 

(d) Intense metabolic activity phase

1

2.

Name the type of animal cell culture which is prepared by inoculating directly from the parental tissue to nutrient media? 

(a) Primary cell culture 

(b) Secondary cell culture 

(c) Spinner culture 

(d) Transformed cell culture

1

3.

Relationship between the number of genes and proteins is not linear because of- 

(i) Alternative splicing of genes 

(ii) Overlapping genes 

(iii) Post translational modification 

(iv) Amplified DNA templates 

(a) (i) (ii) and (iii) only 

(b) (ii) and (iv) only 

(c) (i) and (iv) only 

(d) (ii), (iii) and (iv) only

1

4.

Which of the following statements is incorrect about Agar? 

(a) It is a polysaccharide . 

(b) It is obtained from red algae. 

(c) It is present in both liquid and solid medium 

(d) Solidifying a

1

5.

An example of secondary metabolites produced by microbial cells is: 

(a) Vitamins 

(b) Alcohol 

(c) Amino Acids 

(d) Antibiotics

1

6.

YEp contains a gene coding for: 

(a) X-Gal 

(b) Β-galactosidase 

(c) Leucine 

(d) GFP

1

7.

Reciprocal translocation between chromosome 9 and chromosome 22 forms- 

(a) an extra-short chromosome 9 (der9) 

(b) an extra-long Philadelphia chromosome (Ph1) 

(c) Philadelphia chromosome (Ph1) with fused abl-bcr gene 

(d) der 9 chromosome with fused abl-bcr gene

1

8.

Which of the following statements on differences between homologues and paralogs are correct? 

(i) Homologues have the same function whereas paralogs may differ in function. 

(ii) Paralogs have the same function whereas homologues may differ in function. 

(iii) Homologues are the sequences descended from a common ancestor whereas paralogs are duplicated genes within a genome. 

(iv) Paralogs are the sequences descended from a common ancestor whereas homologues are duplicated genes within a genome. 

(a) (i) and (iii) 

(b) (ii) and (iv) 

(c) (i) and (iv) 

(d) (ii) and (iii)

1

9.

Severe combined immunodeficiency disease is caused due to the absence of: 

(a) Adenosine diphosphate 

(b) Adenosine deaminase 

(c) Adenosine cyclase 

(d) Guanidine nitrate

1

10.

A protein ion with a molecular weight of 10,000 Daltons carried a charge of 5+ and was subjected to mass spectrometric analysis. Calculate its mass to charge ratio. 

(a) 2001 

(b) 2000 

(c) 2501 

(d) 5001

1

11.

Single nucleotide polymorphisms usually occur in __________ regions. 

(a) Mini-satellite only 

(b) Non-coding only 

(c) Regulatory only 

(d) Both Coding and non-coding

1

12.

Which of the following is not a function of serum? 

(a) Cell proliferation 

(b) Promote healthy growth of cells 

(c) Enhance cell attachment 

(d) Provide sterile environment to cell culture

1

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