BIOTECHNOLOGY
CLASS XII
(THEORY)
| One Paper | Time : 3 Hours | Marks : 70 |
|---|
| Marks | ||
|---|---|---|
| Unit V: Protein and Gene Manipulation | 40 | |
| Chapter I: Protein Structure and Engineering | 15 | |
| Introduction to the world of Proteins | ||
| 3-D Shape of Proteins | ||
| Structure Function relationship in Proteins | ||
| Purification of Proteins | ||
| Characterization of Proteins | ||
| Protein based products | ||
| Designing Proteins | ||
| Proteomics | ||
| Chapter II: Recombinant DNA Technology | 15 | |
| Introduction | ||
| Tools of rDNA Technology | ||
| Making Recombinant DNA | ||
| DNA Library | ||
| Introduction of Recombinant DNA into host cells | ||
| Identification of recombinants | ||
| Polymerase Chains Reaction (PCR) | ||
| DNA Probes | ||
| Hybridization Techniques | ||
| DNA Sequencing | ||
| Site-directed mutagenesis | ||
| Chapter III: Genomics and Bioinformatics | 10 | |
| Introduction | ||
| Genome Sequencing Projects | ||
| Gene Prodiction and counting | ||
| Genome similarity, SNP's and comparative genomics | ||
| Functional Genomics | ||
| History of Bioinformatics | ||
| Sequences and Nomenclature | ||
| Information Sources | ||
| Analysis using Bioinformatics tools. | ||
| Unit VI : Cell Culture Technology | 10 | |
| Chapter I: Microbial Culture and Applications | ||
| Introduction | ||
| Microbial Culture Techniques | ||
| Measurement and Kinetics of microbial Growth | ||
| Scale up of microbial process | ||
| Isolation of microbial products | ||
| Strain isolation and Improvement | ||
| Applications of microbial culture technology | ||
| Bio safety of genetically modified micribes | ||
| Chapter II: Plant Cell Culture and Applications | 10 | |
| Introuction | ||
| Cell and Tissue Culture Techniques | ||
| Applications of Cell and Tissue Culture | ||
| Gene Transfer Methods in Plants | ||
| Transgenic Plants with Beneficial Traits | ||
| Diagnostics in Agriculture and Molecular Breeding | ||
| Bio safety of genetically modified plants | ||
| Chapter III: Animal Cell Culture and Applications | 10 | |
| Introduction | ||
| Animal Cell Culture Techniques | ||
| Characterisation of Cell Lines | ||
| Scale-up of Animal Culture Process | ||
| Applicationsof Animal Cell Culture | ||
| Stem Cell Technology | ||
| Bio safety of genetically modified animals |
Practicals
Note: Every student will be required to do the following experiments during the academic session
List of Experiments
1. Isolation of bacterial plasmid DNA and its detection by gel eletrophoresis
2. Restriction digestion of plasmid DNA and its analysis by gel electrophoresis
3. Bacterial transformation using any plasmid
4. Data retrival and data base search using internet site NCBI
5. Download a DNA and protein sequence from internet, analyse and comment on it.
6. Cell viability assay (using Evans blue Stain)
7. Determination of blood groups.
8. Estimation of DNA
9. Ion-exchange chromatography for proteins.
10. Reading of a DNA sequencing gel and arrive at the sequence.
11. Estimation of blood glucuse by enzymatic method (GOD/POD)
12. Project work.
Scheme of Evaluation:
Time: 3 Hours
Max. Marks 30
The scheme of evaluation at the end of the session will be as under:
| A. | Two Experiments : | 6+6 (only one computer bassed practical) |
| Practical record : | 04 | |
| Viva on Practicals : | 04 | |
| B. | Project work : | |
| Write up : | 05 | |
| Viva on project : | 05 | |
| Total | 30 |
Recommended Books:
1. A Textbook of Biotechnology-Class XI: published by CBSE, New Delhi.
2. A Laboratory Manual of Biotechnology-Class XI:published by CBSE, New Delhi.
3. A Textbook of Biotechnology-Class XII: published by CBSE, New Delhi
4. A Laboratory Manual of Biotechnology-ClassXII: published by CBSE, New Delhi.
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