CBSE Class 12 Biotechnology Syllabus 2025-26: Check Course Structure and Subject-wise weightage, Download PDF

CBSE Class 12 Biotechnology Syllabus: The Central Board of Secondary Education (CBSE) has published the Class 12 syllabus for the 2025-26 academic year. Students preparing for the 2026 Class 12 board exams can find the latest Biotechnology syllabus here, outlining the necessary topics. A free PDF download is available.

Jun 5, 2025, 14:57 IST
CBSE Class 12 Biotechnology Syllabus 2025-26 Download PDF Here
CBSE Class 12 Biotechnology Syllabus 2025-26 Download PDF Here

CBSE Syllabus 2025 Biotechnology for Class 12: The Central Board of Secondary Education (CBSE) has published the updated Class 12 syllabus for the 2025-26 academic year. This syllabus, available as free PDFs on the official CBSE Academic website (cbseacademic.nic.in), provides a crucial framework for students and teachers. It details the course structure, content organization, and chapter-wise weightage for the upcoming 2026 board examinations. Access to the latest syllabus at the beginning of the academic session helps in effective preparation. The CBSE Class 12 Biotechnology syllabus for 2025-26 is readily available for download.

CBSE Class 12 Biotechnology Syllabus: COURSE STRUCTURE THEORY

Unit-V Protein and Gene Manipulation 40 Marks

Chapter-1: Recombinant DNA Technology

Introduction, Tool of Recombinant DNA technology, making rDNA molecule, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.

Chapter-2: Protein Structure and Engineering

Introduction to the world of proteins, Structure-function Relationship in proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)

Chapter-3: Genomics, Proteomics and Bioinformatics

Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, Information sources, Analysis using bioinformatics tools.

Unit-VI Cell Culture and Genetic Manipulation 30 Marks

Chapter-1: Microbial Cell Culture and its Applications

Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.

Chapter -2: Plant Cell Culture and Applications

Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Transgenic plants with beneficial traits, Biosafety of transgenic plants

Chapter-3: Animal Cell Culture and Applications

Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.

PRACTICALS 30 Marks

Note: Every student will be required to do the following experiments during the
academic session.
1. Use of special equipment in biotechnology experiments
2. Isolation of bacterial plasmid DNA
3. Detection of DNA by gel electrophoresis
4. Estimation of DNA by UV spectroscopy
5. Isolation of bacteria from curd & staining of bacteria
6. Cell viability assay using Evan’s blue dye exclusion method
7. Data retrieval and database search using internet site NCBI and download a
DNA and protein sequence from internet, analyze it and comment on it
8. Reading of a DNA sequencing gel to arrive at the sequence
9. Project work

Prescribed Books:

1. A Text Book of Biotechnology - Class XI : Published by CBSE, New Delhi
2. As reference- Biotechnology - Class XI : Published by NCERT, New Delhi
3. A Laboratory Manual of Biotechnology - Class XI : Published by CBSE, New Delhi
4. A Text Book of Biotechnology - Class XII : Published by CBSE, New Delhi
5. A Laboratory Manual of Biotechnology - Class XII : Published by CBSE, New
Delhi

To downoad complete Biotechnology class 102 syllabus for the academic yearf 2025-26. Studnts can click on the below mentioned link and download the full PDF for free.

CBSE Class 12 Biotechnology Syllabus 2025-26 Downlaod PDF

The CBSE Class 12 Biotechnology Syllabus for 2025-26 provides a clear roadmap for students preparing for their 2026 board exams. The detailed syllabus helps in understanding the course structure, subject-wise weightage, and chapter-wise breakdown, facilitating a well-organized study plan and successful academic outcomes.

Anisha Mishra
Anisha Mishra

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