CBSE Class 12 Biotechnology Sample Paper 2025: Practise for Last Minute Revision, Download PDF

Mar 21, 2025, 09:58 IST

The CBSE Class 12 Biotechnology Sample Paper 2025 is now available for download here. Students can access the PDF format of the sample paper to enhance their preparation and improve their understanding of key concepts. 

CBSE Class 12 Biotechnology Sample Paper 2025: Download PDF
CBSE Class 12 Biotechnology Sample Paper 2025: Download PDF

CBSE 2025 Class 12 Biotechnology Sample Paper : The Central Board of Secondary Education (CBSE) Senior Secondary Class 12 Biotechnology exam is on 21st March,2025. Therefore, in this article, Students can directly access the Class 12 Biotechnology syllabus with the sample papers of Biotechnology. In order to adequately prepare for the test, you must obtain the most recent sample paper. For effective revision and assessment of preparation level, sample papers should ideally be completed in the final month of preparation. Regularly answering the questions will also help you become more accurate and faster.The link to download the syllabus in PDF format has also been attached at the end of this article.

CBSE Class 12 Biotechnology syllabus 2024-25: 

Check the chapter-wise CBSE Class 12 Biotechnology syllabus 2025 from the table below:

Unit-V Protein and Gene Manipulation (40 Marks)
Chapter-1: Recombinant DNA TechnologyIntroduction, Tool of Recombinant DNA technology, making rDNA molecule, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.
Chapter-2: Protein Structure and EngineeringIntroduction to the world of proteins, Structure-function Relationship in proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)
Chapter-3: Genomics, Proteomics and BioinformaticsGene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, Information sources, Analysis using bioinformatics tools.
Unit-VI Cell Culture and Genetic Manipulation (30 Marks)
Chapter-1: Microbial Cell Culture and its ApplicationsIntroduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.
Chapter -2: Plant Cell Culture and ApplicationsIntroduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Transgenic plants with beneficial traits, Biosafety of transgenic plants
Chapter-3: Animal Cell Culture and ApplicationsIntroduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.

CBSE Class 12 Biotechnology Sample Paper 2024-25: Exam Pattern

Board  CBSE
Subject Biotechnology Class 12
Exam Timing 10:30 am to 1:30 pm
Time / Duration 3 Hours
Total Marks 100
Theory Marks 70
Practical Marks 30
Total Questions 33
Total Sections 5

CBSE Class 12 Biotechnology Sample Paper 2024-25: General Instructions

The General Instructions of CBSE Biotechnology Class 12 is given below: 

(i) All questions are compulsory. 

(ii) The question paper has five sections and 33 questions. 

(iii) Section–A contains 12 Multiple choice questions and 4 Assertion-Reasoning based questions of 1 mark each; Section–B has 5 short answer questions of 2 marks each; Section –C has 7 short answer questions of 3 marks each; Section-D has two case-based question of 4 marks; Section-E has three long answer questions of 5 marks each. 

(iv) There is no overall choice. However, internal choices have been provided in some questions. A student has to attempt only one of the alternatives in such questions. 

(v) Wherever necessary, neat and properly labeled diagrams should be drawn. 

CBSE Class 12 Biotechnology Sample Paper 2024-25: Download PDF

To help you prepare for the test, the most updated PDF of the CBSE Class 12 Biotechnology Sample Paper 2025 is provided below:

Section - A Q. No. 1 to 12 are multiple choice questions. Only one of the choices is correct. Select and write the correct choice as well as the answer to these questions.

1 An approach that utilises analysis of genome present in different environment to screen presence of variety of molecules. 

  1. Metagenomics 
  2. Proteomics 
  3. Strain preservation 
  4. Mutant selection  

2 In animal cell culture, the CO2 levels in the incubator is usually maintained at

  1. 1-2% 
  2. 3-4% 
  3. 5-10% 
  4. 11-15% 

3 Even if we know where the genes are in each genome, it’s difficult to count them due to: 

(i) splice variants 

(ii) overlapping genes 

(iii) Post – trancriptional events 

(iv) Mutations 

  1. (i), (iii) 
  2. (i), (ii) 
  3. (iii), (iv) 
  4. (ii), (iv) 

4 Avocado growers in Kerela want to find a way to genetically engineer their crop to prevent it from ripening during shipping. This can be achieved by introducing

  1. genes for carotenoid biosynthesis. 
  2. gene from Bacillus amyloliquefaciens. 
  3. ethylene forming gene(s). 
  4. gene for protein containing sulphur rich amino acids. 

5 Microbial species used for commercial production of citric acid is

  1. Saccharomyces cerevisiae 
  2. Aspergillus niger 
  3. Streptomyces griseus 
  4. Aspergillus oryzae 

6 To reduce the possibility of relegation of vector without picking insert, the following method or methods may be used- 

(i) Use of DNA Ligase 

(ii) Use of Alkaline Phosphatase 

(iii) Use of two different Restriction enzymes for cloning 

(iv) Hybridoma technology 

Select the correct answer from options below 

  1. (i), (iv) 
  2. (ii), (iii) 
  3. (iii), (iv) 
  4. (ii), (iv) 

7 In a comparative cDNA microarray, the cDNA obtained from the normal and diseased cells are labelled with red and green fluorescent dyes respectively. The yellow colour of the spot indicates

  1. Gene is expressed only in normal cells. 
  2. Gene is not expressed either in normal cells or in diseased cells. 
  3. Gene expressed only in diseased cells. 
  4. Gene expressed in equal measure in both types of cells. 

8 Which disease has its locus on chromosome no 1 and 6? 

  1. Huntington's disease 
  2. Migraine 
  3. Alzheimer’s disease 
  4. Cystic fibrosis 

9 A molecular disease which leads to increased hydrophobic interactions within the haemoglobin protein is

  1. Thalassemia 
  2. Sickle Cell anaemia 
  3. Mad Cow 
  4. SCID 

10 Interleukin-2 produced using CHO cell line is used for

  1. anaemia therapy. 
  2. cancer therapy. 
  3. stroke. 
  4. treatment of autoimmune disease. 

11 Proteome of a given cell is dynamic because: 

  1. In response to internal and external changes the biochemical machinery of the cell could be changed. 
  2. In response to internal and external changes the biochemical machinery of the cell could not be changed. 
  3. No direct relationship exists between internal and external changes in the biochemical machinery of the cell. 
  4. Indirect relationship exists between internal and external in changes the biochemical machinery of the cell.
Apeksha Agarwal
Apeksha Agarwal

Content Writer

Apeksha Agarwal, a passionate and aspiring journalist, is dedicated to delivering impactful stories and insightful reports. As an education beat writer, she focuses on providing well-researched and engaging news content. Apeksha's strong foundation in journalism and media is complemented by her creativity, dedication, and attention to detail. Her goal is to inform and inspire audiences through meaningful narratives while continuously adapting to the ever-changing media landscape. She can be reached at apeksha.agarwal@jagrannewmedia.com.

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