CBSE Class 12 Biology Exam 2025: The Central Board of Secondary Education (CBSE) board exams for higher and secondary classes are ongoing. The exams started on February 15, 2025, for both classes. The exams for two of the major subjects for science students have already been done, which include Physics and Chemistry. Next comes Biology which is scheduled for March 25, 2025. Utilise this time and prepare for the exam effectively. To streamline your exam preparation, Jagran Josh has provided the sure-shot questions for the CBSE 12th Biology 2025 exam. There are high chance that you will see similar questions and thus get ready in advance.
Check | CBSE Board Exam Dates 2025
CBSE 12 Biology Sure Shot Questions For 2025 Exam
If you have solved enough sample papers and previous year papers, then you can judge that the majority of the repeated questions that were asked in almost all the question papers are from three major units: genetics and evolution, biotechnology, and ecology. Thus, to prepare you well for the CBSE 12th Biology exam 2024-25, here is the list of questions that will most probably be asked in the upcoming board exams. The question language might differ, but the concepts will remain the same. Go through and have a nice exam preparation.
Unit-VII: Genetics and Evolution
Principles of Inheritance and Variation
- Explain Mendel’s laws of inheritance with suitable examples.
- What is incomplete dominance? Explain with an example.
- Define linkage and recombination. How do they affect inheritance?
- Discuss the chromosomal theory of inheritance.
- Solve a dihybrid cross using a Punnett square.
Molecular Basis of Inheritance
- Describe the structure and function of DNA with a well-labelled diagram.
- Explain the process of DNA replication.
- What is transcription? Describe the process in prokaryotes.
- Explain the lac operon model of gene regulation.
- Differentiate between exons and introns.
Evolution
- Differentiate between homologous and analogous organs with examples.
- Describe Darwin’s theory of natural selection.
- Explain Hardy-Weinberg principle and its significance.
- What are the different evidences supporting evolution?
- Discuss adaptive radiation with an example.
Check | CBSE Class 12 Biology Competency Based Questions 2025
Unit-IX: Biotechnology and Its Applications
Microbes in Human Welfare
- Name any four microorganisms and their industrial uses.
- Explain the role of microbes in sewage treatment.
- How do microbes help in biocontrol of pests? Give examples.
- Discuss the importance of biofertilizers in sustainable agriculture.
- Explain the production of biogas using microbes.
Biotechnology - Principles and Processes
- Describe the steps involved in recombinant DNA technology.
- Explain the role of restriction enzymes in genetic engineering.
- What is PCR? Describe its application.
- Discuss the role of plasmids in genetic engineering.
- Differentiate between cDNA and genomic DNA.
Biotechnology and Its Applications
- What is gene therapy? How is it beneficial?
- Explain the role of genetically modified organisms (GMOs) in agriculture.
- Discuss the ethical concerns related to biotechnology.
- How are transgenic animals useful in research?
- What are bio-patents? Discuss their significance.
Unit-X: Ecology and Environment (Excluding Deleted Topics)
Biodiversity and Conservation
- Define biodiversity and its types.
- Explain the significance of biodiversity hotspots.
- What are the major causes of biodiversity loss?
- Describe different methods of conservation of biodiversity.
- Explain the role of sacred groves in biodiversity conservation.
Environmental Issues
- Describe the causes and effects of global warming.
- Explain the process and impact of eutrophication.
- What are the advantages of using CNG over petrol and diesel?
- Discuss measures to control air pollution.
- How do ozone-depleting substances affect the environment?
Practical-Based Important Questions (Excluding Deleted Experiments)
- Describe the Mendelian ratio observed in pea plants.
- Discuss the mechanism of pollination in different types of flowers.
- Explain the procedure for isolating DNA from plant material.
CBSE Class 12 Biology Deleted Topics for 2025 Board Exam
Students should go through the CBSE 12th deleted topics before solving these questions or preparing for the exam. This will save your time and efforts. Check here the list of topics which are now deleted or removed from the CBSE Class 12 Biology syllabus 2025.
| Unit & Chapter | Deleted Topic |
| Unit-VI Reproduction Chapter-1: Reproduction in Organisms | (whole chapter deleted) |
| Unit-VIII Biology and Human Welfare Chapter-9: Strategies for Enhancement in Food Production | (whole chapter deleted) |
| Unit-X Ecology and Environment Chapter-13: Organisms and Populations | Organism and its Environment, Major Aboitic Factors, Responses to Abioitic Factors, Adaptations |
| Unit-X Ecology and Environment Chapter-14: Ecosystem | Ecological Succession and Nutrient Cycles |
| PRACTICALS A. List of Experiments | 2. Collect and study soil from at least two different sites and study them for texture, moisture content, pH and water holding capacity. Correlate with the kinds of plants found in them. 3. Collect water from two different water bodies around you and study them for pH, clarity and presence of any living organism. 4. Study the presence of suspended particulate matter in air at two widely different sites. 8. Study the effect of different temperatures and three different pH on the activity of salivary amylase on starch. |
| PRACTICALS B. Study/observation of the following (Spotting) | 10. Two plants and two animals (models/virtual images) found in xeric conditions. Comment upon their morphological adaptations. 11. Two plants and two animals (models/virtual images) found in aquatic conditions. Comment upon their morphological adaptations. |
Keep revising and stay positive, this is going to support you a lot through the board examination.
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