CBSE Class 12 Biotechnology Syllabus 2019-20

CBSE Syllabus for Class 12 Biotechnology subject is available here for download in PDF format. Link to download CBSE Class 12 Biotechnology Syllabus 2019-20 is given at the end of this article. Besides the syllabus, here you will also get complete details about practical is CBSE Class 12 Biotechnology subject for the session 2019-20.

Important portion of CBSE Class 12 Biotechnology Syllabus 2019-20 is given below

Unit-V Protein and Gene Manipulation (40 Marks)

Chapter-1: Recombinant DNA Technology

Introduction, Tool of DNA technology, Making DNA, Introduction of recombinant DNA into host cells, Identification of Recombinants, Polymerase Chain Reaction (PCR), Hybridization Techniques, DNA Library, DNA Sequencing, Site-directed Mutagenesis

CBSE Class 12 Syllabus 2019-20: All Subjects

Chapter-2: Protein Structure and Engineering

Introduction to the world of proteins, 3-D shape of proteins, Structure-Function Relationship in Proteins, Purification of Proteins, Characterization of Proteins, Protein Based Products, Designing Proteins (Protein Engineering)

Chapter-3: Genomics, Proteomics and Bioinformatics

Introduction, Genome, Sequencing Projects, Gene prediction and counting, Genome Similarity, SNPs and

Comparative Genomics, Functional Genomics, Proteomics, History of Bioinformatics, Sequences and nomenclature, Information Sources, Analysis using Bioinformatics tools

Unit-VI Cell Culture and Genetic Manipulation (30 Marks)

Chapter-1: Microbial Cell Culture and its Applications

Introduction, Microbial Nutrition and Culture Techniques, Measurement and Kinetics of Microbial Growth, Scale-up of Microbial Process, Isolation of Microbial Products, Strain Isolation and Improvement, Applications of Microbial Culture Technology, Biosafety Issues in Microbial Technology

Chapter -2: Plant Cell Culture and Applications

Introduction, Cell and Tissue Culture Techniques, Applications of Cell and Tissue Culture, Gene Transfer Methods in Plants, Transgenic Plants with Beneficial Traits, Biosafety of Transgenic Plants

Chapter-3: Animal Cell Culture and Applications

Introduction, Animal Cell Culture Techniques, Characterization of Cell lines, Methods of Gene Delivery into Cells, Scale-up of Animal Culture Process, Applications of Animal Cell Culture, Stem Cell Technology, Tissue Engineering

Syllabus of CBSE Class 12 Biotechnology Practicals 2019-20 (30 Marks)

Note: Every student will be required to do the following experiments during the academic session.

1. Use of special equipment in biotechnology experiments

2. Isolation of bacterial plasmid DNA

3. Detection of DNA by gelelectrophoreses

4. Isolation of Genomic DNA (CTAB method)

5. Estimation of DNA

6. Bacterial transformation using any plasmid

7. Restriction digestion of plasmid DNA & its analysis by gelelectrophoresis

8. Isolation of bacteria from curd & staining of bacteria

9. Cell viability assay

10. Data retrieval and database search using internet site NCBI and download a DNA and protein sequence from internet, analyze it and comment on it

11. Reading of a DNA sequencing gel to arrive at the sequence

12. Project work

Scheme of Evaluation: Practicals (Time: 3 Hours, Max. Marks 30)

Prescribed Books for CBSE Class 12 Biotechnology:

• A Text Book of Biotechnology - Class XII: Published by CBSE, New Delhi

• A Laboratory Manual of Biotechnology - Class XII: Published by CBSE, New Delhi

Download CBSE Class 12 Biotechnology Syllabus 2019-20 in PDF Format